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The theory of your cTnT measurement is really an ELISA just one-step sandwich assay working with streptavidin know-how and electrochemiluminescence detection.[fifty four] In the primary incubation action (immunological reaction), the cTnT through the sample reacts which has a biotinylated mouse monoclonal anti-cTnT antibody as well as a monoclonal
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Even more, the most stable areas of cTn are picked as epitopes; regions that are not prone to cleavage or article-translational modifications, e.g. phosphorylation.16 The detection antibody is connected to a signal-generating program to enable quantification. Sign amplification is accomplished by utilizing an enzyme which may cleave several molecul